Balanced Vav2 GEF activity regulates neurite outgrowth and branching in vitro and in vivo
M Moon, TM Gomez - Molecular and Cellular Neuroscience, 2010 - Elsevier
We have investigated the role of Vav2, a reported Rac1/Cdc42 GEF, on the development of
Xenopus spinal neurons in vitro and in vivo. Both gain and loss of Vav2 function inhibited
the rate neurite extension on laminin (LN), while only GFP-Vav2 over-expression enhanced
process formation and branching. Vav2 over-expression protected neurons from RhoA-
mediated growth cone collapse, similar to constitutively active Rac1, suggesting that Vav2
activates Rac1 in spinal neurons. Enhanced branching on LN required both Vav2 GEF …
Xenopus spinal neurons in vitro and in vivo. Both gain and loss of Vav2 function inhibited
the rate neurite extension on laminin (LN), while only GFP-Vav2 over-expression enhanced
process formation and branching. Vav2 over-expression protected neurons from RhoA-
mediated growth cone collapse, similar to constitutively active Rac1, suggesting that Vav2
activates Rac1 in spinal neurons. Enhanced branching on LN required both Vav2 GEF …
